Bimolecular Fluorescence Complementation (BiFC) Assay for Direct Visualization of Protein-Protein Interaction in vivo
نویسندگان
چکیده
منابع مشابه
MONITORING iN ViVO PROTEIN-PROTEIN INTERACTIONS BY COUPLING BIMOLECULAR FLUORESCENCE COMPLEMENTATION (BIFC) AND FLOW CYTOMETRY
We present a high-throughput approach to study weak protein-protein interactions by coupling bimolecular fluorescent complementation (BiFC) to flow cytometry (FC). Bimolecular Fluorescence Complementation (BIFC) is a method to detect proteinprotein interactions based on the formation of a fluorescent complex by fragments of the yellow fluorescent protein (NYFP and CYFP) brought together by the ...
متن کاملBimolecular fluorescence complementation analysis system for in vivo detection of protein-protein interaction in Saccharomyces cerevisiae.
The bimolecular fluorescence complementation (BiFC) assay has been widely accepted for studying in vivo detection of protein-protein interactions in several organisms. To facilitate the application of the BiFC assay to yeast research, we have created a series of plasmids that allow single-step, PCR-based C- or N-terminal tagging of yeast proteins with yellow fluorescent protein fragments for Bi...
متن کاملPhotoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM) for Nanoscale Imaging of Protein-Protein Interactions in Cells
Bimolecular fluorescence complementation (BiFC) has been widely used to visualize protein-protein interactions (PPIs) in cells. Until now, however, the resolution of BiFC has been limited by the diffraction of light to ∼250 nm, much larger than the nanometer scale at which PPIs occur or are regulated. Cellular imaging at the nanometer scale has recently been realized with single molecule superr...
متن کاملBimolecular fluorescence complementation (BiFC) analysis of protein interactions in live cells.
Bimolecular fluorescence complementation (BiFC) analysis enables direct visualization of protein interactions and modifications in living cells. It is based on the facilitated association of two nonfluorescent fragments of a fluorescent protein fused to putative interaction partners. The intrinsic fluorescence of the active complex enables detection of protein interactions with high sensitivity...
متن کاملBimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells.
Protein interactions are a fundamental mechanism for the generation of biological regulatory specificity. The study of protein interactions in living cells is of particular significance because the interactions that occur in a particular cell depend on the full complement of proteins present in the cell and the external stimuli that influence the cell. Bimolecular fluorescence complementation (...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2013
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.935